FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6504248
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT19600.065.479119
CGCGATT9950.053.2759939
GCGGATA14550.052.2521979
CGCGTTA4500.051.153567
TGCGTTA6000.051.1535577
CGGGATT24900.050.9815569
CGGTATT17800.050.548799
CGCGAGT10350.049.86929
GCGTAAT12450.049.8610849
GGCGGAT21900.046.5007328
CGTTATT14650.045.706159
TCGCGAG5600.044.839998
ACGGATT14100.043.036929
GGGTATT49650.042.9876029
GGGGATT87600.042.677779
GCGGGAT24850.042.383978
GGCGATT29550.042.250969
GCGCATA11450.042.0324449
CGATCGA4150.042.0209927
GCGTATT20750.041.6816259
Produced by FastQC (version 0.11.5)