FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9989652
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT49100.082.351079
CGGGATT55050.076.422969
GGCGATT50300.075.506319
CGCGATT22350.074.1352549
GGGGATT161950.064.796829
CGCAGAC74400.064.522793
GGGTATT83200.063.7605869
CGGTATT31050.063.464719
GCGCATT33200.062.846259
GCGTATT36750.059.3729559
GACGATT20350.059.306859
GGCGGAT48150.054.662748
GCGTAAT22100.053.993549
ACGGATT30800.053.5748149
GCGGGAT46900.053.357278
CCGCATT20300.053.0709769
GGGCATT105300.053.0336579
CGCGAGT21350.052.3771679
CCGCAGA92900.052.3378072
CGTGATT28250.051.4111339
Produced by FastQC (version 0.11.5)