FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6924032
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT26800.068.183439
CGGGATT30100.060.708189
CGCGATT10800.059.923569
ACGGATT13550.059.0000539
CGCGTTA3800.056.77017
GGGGATT83550.055.8164379
CGGTATT20900.054.644629
GGCGATT27300.052.7575239
TCGCGAG6450.052.1363338
GCGGGAT23600.051.081778
GGGTATT54050.050.8292129
GGCGGAT25700.045.673368
GACGATT12350.044.1827439
CGATATT17500.042.7824029
GCGTATT23150.042.7557759
GCGGTAT14250.042.7445458
ACGCGAT4850.041.8632168
GCGTAAT12800.041.638069
GTGCGGA20600.041.5806127
CGTCGAC7950.041.5011868
Produced by FastQC (version 0.11.5)