FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7505546
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT30850.068.3258369
CGCGATT12250.061.3062259
CGGGATT35400.060.9700439
GGGGATT91050.055.03449
CGGTATT23750.055.0047769
GGCGATT29300.054.7091269
ACGGATT17650.053.276459
GGGTATT51850.051.850699
GCGGGAT29100.051.1814658
GCGTATT26250.050.487489
GACGATT12850.050.0945789
GCGGTAT16500.048.9575588
GCGGATA18300.045.52159
CGCGAAA12900.045.4974379
GCGTAAT16650.045.484229
GGCGAAT25500.045.0427479
GGTGCGA25150.044.9167947
GGCGGAT31600.044.5360268
CGCGTTA5150.044.1152737
TCGCGAG6800.043.6196948
Produced by FastQC (version 0.11.5)