FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6775196
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG90380.13339835482250254No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA86810.1281291345667343No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA70500.10405603026096959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG2400.081.8447341
ACCCGTT5400.080.214017
CGATCGT2050.079.27606145
CGTCGAC7850.073.284698
CGCCGTA3750.068.582977
GCTAGCC17350.063.6344221
TCGTGTT19100.059.2085464
CGGCGAT5200.058.600872
TTCGTTA6950.057.4864235
GTATGCG3300.057.4568985
CGTATCG4400.057.082035145
GTTCGCG2850.057.024893
AGCGTGA15700.055.2088136
GCGTGAT15750.055.003897
ACGTAAC7450.054.5078437
CGTCCTA5500.053.724274145
TCGTTAG4300.053.543726
TTACGCG3800.053.4608356
GTCGACC11350.050.685899
CTTTCGT22400.050.497791
Produced by FastQC (version 0.11.5)