FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6775196
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA4800.055.5984957
GCGGATT20100.054.8527769
CGCGATT12550.052.0396359
TCGCGAG6500.051.8596158
CGGTATT20150.047.746399
CGGGATT29150.047.4594469
GCGTAAT13500.045.776649
CGTGATT19550.045.619659
GGGTATT47900.044.715639
GTCGCGA9650.043.6661157
CGCGATA7800.043.2157069
GCGGGAT25700.043.174318
GCGTATT22300.043.1430369
GCGGATA17650.042.572989
CGCGAGT13200.042.0289159
GGCGGAT25500.041.3097468
CGTTATT15300.040.8501439
CGCGGTA9450.040.1312457
CGCGCTA6300.040.131247
TCGCGTA5150.039.5468067
Produced by FastQC (version 0.11.5)