FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8871685
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA165330.18635693219495508No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG148720.1676344459930667No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA117960.13296234029950343No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG100080.11280833347892762No Hit
AACAAGTTGCAGGGAAGTCTTAAGAGAGCCGCCCGAAGCCCCGCACCTCC92350.10409521979195609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT11000.0111.3683557
GTTAGCG2600.070.299071
CGCACTA5550.069.525011
CGTCGAC7750.068.1183248
GTACCCG18350.067.139765
GCTAGCC29200.066.536231
TCGTGTT30300.065.234884
CGCCGTA4900.060.776067
CGGCGAT5050.060.3195762
CGAACGT3450.058.8655661
TACCCGT21100.058.3893246
CGTATCG5750.057.67139145
CTTTCGT34750.057.468041
GTATGCG4500.057.1630745
CCGCGTT3800.057.0040864
CGGTATA11000.056.0006646
CGCGATA5650.055.104287
CGGGTTA15350.054.2420774
TATTGCG6900.052.968867
GCTTAGA49600.052.8170056
Produced by FastQC (version 0.11.5)