FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8871685
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT35550.068.811659
CGCGATT13700.067.2434849
GGCGATT35150.060.032089
GGGGATT115000.059.8099569
GGGTATT63450.059.1554459
CGGTATT27450.057.823839
CGGGATT38100.055.0571759
ACGGATT18900.055.000139
GCGTATT29450.054.5319
GCGGTAT18800.053.6375168
CGTTATT23200.053.391759
GGCGGAT32300.052.417698
GACGATT16100.051.8069429
GCGCATT28150.047.541099
TCGCGAG6400.046.68458
CGCGAAT13100.046.0902949
TAGCGAG8750.045.5285078
GGGCATT67650.044.809519
CGATATT24400.044.64349
GGCGAAT33200.044.246919
Produced by FastQC (version 0.11.5)