FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6748812
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT20000.065.502119
CGCGATT11050.058.6405339
CGCGTTA6050.057.04617
GTCGCGA10050.054.6655547
CGGGATT28800.053.5579579
GCGTAAT13600.053.342179
CGCGATA9750.052.0116049
CGCGGTA9400.047.206087
GGCGGAT21750.045.9841238
CGTTATT18000.045.7810489
TCGCGAG6400.045.1214148
GCGGGAT25700.044.9458438
CGCGAGT11150.044.8493169
GGCGATT33400.044.494719
GCGTATT21250.043.419499
GTATCGA9100.041.7962767
GCGTCGA11600.041.2891737
GCGGATA16300.041.0495539
CGTTAAT14450.040.9433949
GCGTATA12650.040.6447759
Produced by FastQC (version 0.11.5)