FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156790
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG30040.25968412590012013No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA23540.2034941519204004No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA21230.18352509962914618No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG19390.16761901468719476No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC18500.15992531055766387No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG15440.13347279973028814No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG14040.12137034379619464No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC13730.11869051426793109No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC13350.11540556194296284No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA13020.11255284018706939No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA12140.10494558217135348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG254.4918124E-8140.440646
ACCCGTT700.0120.3776867
CGATCGT758.185452E-11113.33013145
CGCGTAG353.341156E-7100.3147356
CGATTAG555.366019E-1089.371311
GTTAGCG407.399467E-787.77541
CTTTCGT4150.084.602781
CGAACGT250.001065724584.264381
CGTCGAC1550.081.546178
CGAGTTA950.081.307747
CCGCGTT354.0276624E-580.251794
CCGATAC1500.079.583028
GTTCGCG451.4909692E-678.0225753
TTCGCGC451.4909692E-678.0225754
GCTAGCC4050.076.2887341
TCGTGTT4650.075.5057144
AACGATT2100.073.564149
ACGCGCC502.7884762E-670.220328
CGCGTTC407.806199E-570.2203145
CGCCGTA407.806199E-570.2203147
Produced by FastQC (version 0.11.5)