FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7211308
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT11650.073.263179
GCGGATT27400.066.881359
GGGGATT82000.059.6971329
CGGGATT31100.059.3279889
ACGGATT15000.058.5746239
GGCGATT26150.057.5986449
GCGGTAT13700.056.3452458
GGGTATT50800.055.8402569
GCGTATT19550.052.6465389
GCGGGAT24250.050.7244158
CGTTATT19050.049.086729
ACGCGAT4750.048.8855868
CGCGATA6550.048.8654149
GGCGTAT13850.048.4849248
GGCGGAT26000.048.0344128
GACGATT11200.047.068899
CGGTATT28400.046.4059529
CGCGTAA7600.046.2431228
GCGGATA14800.045.796769
GGCGAAT25400.044.96879
Produced by FastQC (version 0.11.5)