FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6883383
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAATGCCAG225960.32826881781821526No Hit
TTTCCCTTCTGGTATCAAAATGCTCCGGAGAGGAGGGACTGTCAGGGGAG213590.3102980031766357No Hit
TTGGCTTAGTCTTGCTCTTATAAAGGCTTCCGGGTCATGGCACCTGGGAA185800.26992541312898033No Hit
AACAAGTTGCAGGGAAGTCTTAAGAGAGCCGCCCGAAGCCCCGCACCTCC181080.26306831974916983No Hit
GCCGGATGGAGTTGTCGGTGTAGATGCACAGCTTCTCGGCCGTCAGGGGG170500.24769797060544213No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG155030.22522355649830902No Hit
GCGTGTTTGCGGATGATCTGTTTGTTCTCCTCCGCCTCTGGCATTTTGGA151280.2197756539190105No Hit
GGCACTAGGTGTCTCCCCCTGTAAGCCCCGGCAAGGCTGCCTGGGACATC142710.20732538055778676No Hit
TGCAGGCGGCTCTTTTTCACGGGCTCCAGCGACAGGAAGCGGTCCAGGTA141490.2055529962519883No Hit
GCACACATTTGAAGTAGGACACCGAGGGCGCGCAGGTCTCCTCCGCCTTC140460.20405663901020762No Hit
CCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCATCCCCCTGACG135580.19696710178701374No Hit
CCCCACCCTCTCTCTCTCAGGACTGCCTCCGGGCCTGCCAGGAGCAGATC117190.17025058753813352No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG111750.1623474968630977No Hit
GAGGAAGCGTGTGAGGCGGTAGTAGGACAGGAAGTTGTTGGGGCTCCTCA109450.15900611661446123No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA103460.15030400022779497No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA100070.14537909629610907No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG96290.13988761049617607No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC90910.13207168626240903No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC89820.13048816257935958No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA78080.11343259557110216No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC70650.10263848459398524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT3100.094.9536741
CGCAATG42100.091.2268451
CTTAGTC33650.087.6618655
GTGAGCG30550.087.44075145
GTTAGCG1650.084.951341
ACCCGTT6500.084.030347
GTTTGCG31600.083.370885
GCTTAGT37250.079.564884
TTACGCG4000.075.322056
GCGTGTT36850.074.9346851
GCCGGAT35150.074.770471
GTTCGCG3750.074.737623
GCTAGCC20850.071.597471
CGTCGAC4850.070.74728
TTAGTCT42000.070.400676
CGATCGT2150.070.32861145
CGCGATA3800.070.025287
GACCCCG54850.069.578457
GGCTTAG42850.069.494213
GCACTAG38300.068.968372
Produced by FastQC (version 0.11.5)