FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7616613
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT12650.062.9586529
GCGGATT26200.060.795969
ACGGATT18500.050.2251249
CGGGATT32350.050.0197079
TCGCGAG6150.049.3334588
GGGGATT81200.049.041079
GACGATT13550.048.0477839
GGGTATT49300.047.823069
GTCGCGA8350.047.690247
CGGTATT21900.047.0455869
GGCGATT26550.046.6623889
CGTTATT19550.046.557669
CGCGAAA12000.046.3528949
GCGTATT24500.045.664919
GCGTAAT15600.045.3804559
CGCGTTA4300.044.0989467
GCGGGAT27500.043.901178
CGTGATT21600.043.602159
ACGCGAT5350.042.5328188
GTACGGA11050.041.1856237
Produced by FastQC (version 0.11.5)