FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8241696
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT31800.068.138239
CGGGATT37150.064.090339
CGCGATT13650.062.757539
GGGGATT97600.062.536499
ACGGATT16700.061.8567289
GCGTATT26050.060.6911779
CGGTATT28650.060.020229
GGCGATT29800.059.606339
GCGGTAT18600.058.247568
GGGTATT58600.057.2913639
GCGGGAT30750.052.8490078
GCGTAAT18100.051.5042089
CGTTATT21050.051.467849
GACGATT13700.050.574519
CGCGAAT15300.047.75589
GGCGTAT18950.046.5351378
GGCGAAT29550.045.8290189
GGCGGTA25150.045.331787
GCGGAAT31150.045.2949269
CGCGTTA5450.045.0744367
Produced by FastQC (version 0.11.5)