FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7984893
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG154690.19372833173844659No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA139230.17436676984901364No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA108770.1362197339400791No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC92780.11619441863529041No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC92310.1156058071160127No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG88280.11055877642943994No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT82090.10280663748405895No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA81050.10150417795204017No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG79940.10011405287459706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT10200.0108.535647
CGATCGT2400.086.93218145
GTTAGCG2000.086.505591
GCTAGCC25200.077.717731
CGTCGAC5600.074.1341868
CGCGATA5200.073.183297
TCGTGTT32750.068.461024
CTTTCGT32800.068.360521
CGGCGAT3500.067.222992
CGCCGTA3950.066.563947
CGCACTA4550.065.402031
GTACCCG18200.061.977115
ACGCGTA3800.061.767605145
CGGTATA11850.058.9836656
CGTGTTC38900.057.4603235
TTCGTGT39600.056.7930373
CGAACGT3550.056.5332341
TACCCGT20050.056.260296
TTTCGTG46450.056.0156172
GTTCGCC4150.054.14802140-144
Produced by FastQC (version 0.11.5)