FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6629146
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT20250.062.8599369
GCGTAAT12050.049.5721979
TCGCGAG7400.049.0103348
CGCGTTA4450.047.9431767
CGCGAGT14350.047.077969
CGCGATT10750.046.9673239
TCGCGTA5350.045.195037
GCGGATA14900.044.3856549
GCGTATT22200.042.603439
GGGTATT51600.042.4469959
ACGGATT13300.042.2397549
ACGTAGT11600.041.6866389
CGGGATT28000.040.635719
CGGTATT19900.040.0231139
GGCGGAT23750.039.8232968
GGCGTAT15800.038.2570088
CGGTAGT17750.037.258949
GGGGATT93400.037.1551369
TTCGCTA6000.036.7431227
GGTGCGA20000.036.6245967
Produced by FastQC (version 0.11.5)