FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7969859
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT28050.065.427569
CGGTATT23150.051.3144079
GCGTAAT14650.050.011919
TCGCGAG8500.049.3749858
CGGGATT32900.047.566449
CGCGAGT16050.046.09279
GGCGGAT30100.046.083138
CGCGATA8300.045.422569
GCGGTAT20300.044.5022058
CGCGATT13300.044.391559
ACGGATT17050.044.2237249
GGCGATT37400.044.1255579
GCGGATA20200.042.9617849
GGGGATT111500.042.9352539
GGCGGTA28600.042.7814337
GCGTTAA12100.041.739468
CGCGAAA17000.041.4247749
GCGCATT22850.041.4037139
GGGTATT60550.041.235089
CGCGTTA5900.040.9939587
Produced by FastQC (version 0.11.5)