FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14542151
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC370820.2549966645237008No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG246180.16928719829686817No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA232540.15990756800696126No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG217490.14955834250380154No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC174020.11966592837607036No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG150800.10369855188548105No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC149690.10293525352611178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT21950.081.715157
CTTTCGT51450.077.662551
CTAGTCG40800.076.101493
TAGTCGA43800.071.453914
TCGTGTT58300.068.429094
AGTCGAA46600.067.558725
TTCGTGT61950.064.4971853
AGCGTGA48150.063.4566656
GCGTGAT49400.062.3507277
GTACCCG30050.059.484385
TTTCGTG75950.058.6346472
GTCGAAA53350.058.3149836
CTGTCTA110300.055.911625145
GTTCGCG9650.055.7624053
GTTAGCG5800.054.3884541
ATAGCGT56600.053.9833834
AACGATT20300.053.0198679
TACCCGT34100.052.2377786
CGTCGAC18500.051.825738
CGCGTTC15100.051.6111075
Produced by FastQC (version 0.11.5)