FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14542151
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT56600.070.536299
GGCGATT65650.065.600319
CGGGATT64600.065.113779
CGCGATT26050.062.894569
CGCAGAC92200.060.3441543
GGGGATT220250.059.2374159
CGGTATT39200.059.1967939
GGGTATT109350.056.9969869
CGTTATT31750.050.971389
GCGTAAT25050.050.1885769
GCGCATT47350.049.996329
CCGCATT24450.049.779139
TGCGATT41700.049.3934569
CGTGATT40000.049.1521039
GCGTATT49600.048.9417729
CCGCAGA115150.048.438232
ACGGATT40850.048.293069
TACATTA192250.048.078312145
CGCGAGT23600.046.7548529
GACGATT33400.045.249819
Produced by FastQC (version 0.11.5)