FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9154051
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT37350.072.833389
CGGGATT43250.060.996179
CGCGATT14000.059.6483579
GGGGATT113550.057.942719
GCGTATT28700.056.4300779
CGGTATT32050.056.0586859
GGCGATT36950.053.2474179
CGTTATT22200.052.434549
GCGGGAT34700.051.7783978
GGGTATT72650.051.377039
ACGGATT20700.051.0385979
ACGCGAT5150.050.3658688
GCGGTAT20000.050.2952358
GACGATT14900.049.2520529
CGCGATA7750.048.9781279
CGCGTTA5050.048.8576937
CGCGAAT13250.047.745979
GGCGGAT34550.047.2423368
GGCGTAT18900.046.8625538
GCGTAAT18800.045.4285139
Produced by FastQC (version 0.11.5)