FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6113046
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT19050.054.0994229
CGCGATT10050.053.0413789
GCGGATA16250.050.7369359
CGCGTTA4100.048.5429087
CGCGATA7100.048.051019
CGCGAGT12350.047.7673079
GGGGATT86800.044.299339
TGCGTTA6450.044.0810437
GGGTATT48750.043.8845379
ACGGATT12800.043.866889
ACGCGAT5350.043.8430868
GCGTAAT12650.043.825189
GGCGGAT22300.043.667338
GCGTATA11300.043.4000559
CGGTAGT13600.043.376939
CGGTATT17500.042.2391249
CGGGATT25250.042.223049
CGATCGA4000.040.871397
CGGGATA20400.040.763869
GGGGATA55700.040.5780879
Produced by FastQC (version 0.11.5)