FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7436419
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT29500.067.0666059
CGCGATT10150.065.389399
CGGGATT32500.062.820769
GGGGATT93600.061.318859
GGGTATT60000.056.3620229
ACGGATT15850.055.0343489
GACGATT12600.054.179789
GGCGATT27200.053.4494179
GCGGTAT15250.051.396788
GCGGGAT26000.051.2971348
CGGTATT26750.050.5677959
GCGTATT25450.050.1704069
GGCGGAT28450.049.1014188
CGCGTTA4350.046.4991237
CGCGATA6400.046.419669
CGTTATT18150.045.9707229
GGCGAAT25150.045.490919
GCGTAAT15000.045.089629
CGCGGTA11250.044.949157
TCGCGAG5850.044.3008468
Produced by FastQC (version 0.11.5)