FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350853
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3754345.107352847349825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT20550.055.4512989
CGCGAGT14150.054.142819
CGCGATT9850.047.713029
GCGGAGT34200.045.178889
GCGCAAT12750.043.9298139
TCGCGAG6450.042.9199338
CGCGTTA3900.042.919937
TGCGAGT17950.041.604849
CGCGACT12750.041.4051139
CGGTAGT17300.041.3073359
CGCGAAT9600.040.9080629
GGCGGAT22200.040.5999378
CGGTATT19400.040.486339
CGTTCGA6700.040.357557
CGGGACT23200.040.237449
CGCTAAT9950.040.1161169
CGAGAGT14700.039.854229
GCGTAGT19600.039.744739
GGGGATT93850.039.7186629
GCGGAAT24750.039.5383649
Produced by FastQC (version 0.11.5)