FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6069773
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT22200.060.146899
CGCGATT9550.058.753269
CGCGAGT13350.054.2659239
GTCGCGA9850.054.0853737
CGGTATT17800.053.068889
TCGCGAG7300.052.5404138
CGCGTTA3950.052.150177
GCGTAAT11450.050.864729
CGCGATA7450.049.5741589
ACGGATT11850.046.7503179
GCGGATA16000.045.278139
CGGGATT25750.044.9592069
GGCGGAT24100.043.912878
GCGCATT17750.043.2149169
TCGCGTA4600.041.692777
CGGTAGT18800.041.5568969
GCGTATT21050.041.1638539
GCGTAAA16300.040.9589239
GGTGCGA19850.040.7942667
ACGTAGT10050.040.2825629
Produced by FastQC (version 0.11.5)