FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521685
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTCTTTAAAACGTTTTTATGGTATTTATCCATGATGCTCAAAATTAA89280.25351500773067437No Hit
ATTGAAGGAAATAGGGCGATGAATTGATGCTAATGAATGTGTTTTATTAG88540.25141374086552315No Hit
CCTATAGTTCTGGGATAATTTTGGAAGTATAATAGCAGTTCTTTTCTCTT87380.2481198630769078No Hit
ATCTGAGAGTTCTTGTCCTTCTTCACTAAGGCTATAATCTTCTGAGTCGA85650.24320744189216242No Hit
CCCAGATGGCTTTTTACAATGGACTAAACTGAAGAATTACCTGTGCTCTT81130.23037267671583345No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG79870.22679484394544086No Hit
TCACAAGAAAGTGAAGACTATTCTCAGCCATCAACTTCTAGTAGCATTAT72880.20694639071921536No Hit
TGGCACGCCAAACAAATCTCCTAGAAGATCATTTGAACAATATACAATAT70480.20013147115656285No Hit
TGTATTTTCCACAGATGTTTCATGATTTCCAGTTTTCATCGTGTCTTTTT67400.19138565771782542No Hit
TGCTACAATTGAGGTATACGAAATTTAGTTTATCACTTCATAAAATAAAT67280.1910449117396928No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA66150.18783622044561055No Hit
CTGTAGATTCACTGCTACTGCTTCTTTCACAACATATCTCCCTTATTACA66080.18763745195836654No Hit
GCCCACCACCAAGTTTCTGATCCTTTTTCTTTTCTCTCAGAATCATCGGA64670.18363368671530816No Hit
TCACATCTTCTTGGCTGCTATAAATAATGCTACTAGAAGTTGATGGCTGA61750.17534220124741423No Hit
AAGCACTAATTTCATTAGAGAAAGAATATCAAAAAGCTGTGTGAATGCGT61720.17525701475288108No Hit
AGACTTTGAACAGTAAGAGTCTTGCCTTACTTAAAATGAGAACATTACCG57180.16236545858019671No Hit
CCCCTTCCATCACATTGCAACAGATGTTGGGCCCTTCGTGAGAATTGGCT50230.14263058734668205No Hit
CTTTCTCAGAGATTTCCCCTTTATCTTTCCCTTTATCTTCAGGAAGCCAA50000.14197749088859452No Hit
AAAAGCTGTGTGAATGCGTCAAATAAATATTCATATATACCTGTCTCACT49330.14007499251068736No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA49010.13916633656900035No Hit
CCCCGACTCCAAGCGCGAAAACCCCGGATGGTGAGGAGCAGGTACTGGCC46950.13331686394439027No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC46510.13206746202457065No Hit
CCCAAGACAAAGAAGAGAGTGTGGAATCTAGTTTGCCCCTTAATGCCATT44980.12772295080337964No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG43380.12317967109494461No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA41150.11684747500131329No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC39000.11074244289310373No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG38690.10986218244959443No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC37250.10577323071200291No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA35950.10208181594889947No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG35440.1006336455418358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG1150.0115.82841
ACCCGTT4750.0112.1419757
ACGCGTA2000.095.193085145
CGTCGAC2700.083.068148
TTACGCG1700.082.456176
ATTGCGT3450.081.2611546
CCCGACT9800.077.954742
CGCCGTA1800.073.982567
GTACCCG7250.073.471295
CTTTACG10100.073.044205145
GTTCGCG1650.072.207523
TCGTGTT11600.069.482564
GCTAGCC13150.069.306961
CGATCGT1401.7462298E-1067.99506145
CCGACTC11650.064.970363
CGTTATA1750.064.082049
CCGCGTT1550.063.304164
CTTTCGT13050.062.3170281
TAGGACG2700.062.30118
CCTATAG22000.061.5026551
Produced by FastQC (version 0.11.5)