FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6897768
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC344450.49936443208875686No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG94530.1370443308618092No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG84760.12288032882520837No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA79840.11574758675559978No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG70870.1027433801774719No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA69930.1013806205137662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG5700.082.112293
GTAATAT61500.080.768871
CTTTCGT24000.079.04391
TAATATA64150.078.795942
TTACGCG4550.071.3234946
CGCCGTA5900.069.814937
ACCCGTT7450.069.530967
TCGTGTT27850.069.015614
CGCGTTC7800.068.808885
TAGTCGA15850.068.114354
TTCGTGT29050.066.594343
AGTCGAA16250.066.440585
CCGCGTT8400.063.8911864
CGCGTAG7300.063.2629626
CGTCGAC9400.063.0719768
GCGTGAT21000.062.7075167
GCTAGCC17300.062.0423621
AGCGTGA21250.061.6738436
TTTCGTG33950.061.0258452
GTTAGCG1950.060.8030051
Produced by FastQC (version 0.11.5)