FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6723829
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8650.062.173459
GCGGATT25550.057.4512149
CGGGATT27750.053.124539
GGCGATT23200.049.9074369
CGGTATT20450.049.19339
GGGGATT74150.048.892889
GCGGGAT21400.047.8968168
CGCGTTA4100.047.8391237
GGGTATT48250.045.8957949
GACGATT11300.045.3532839
ACGGATT12450.043.705019
TAGCGAG6400.043.4988828
GTACGGA8600.041.9354937
TCGCGAA5350.041.392318
ACGCGAT4150.041.164338
CGTTATT15800.040.8455969
CGTCGAC8250.040.646898
GCGCATT22600.040.034079
CGCGATA6500.039.909179
GTCGCGA7150.039.8209727
Produced by FastQC (version 0.11.5)