FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3706848
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA108560.29286337071279966No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG72050.19436998765528019No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA61610.16620589784096892No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT50500.13623434249259747No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG48900.13191800688887162No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG47360.1277635338702855No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC41320.11146936696622037No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG41110.11090284791823134No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC40920.11039028306528889No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG39850.10750373363029722No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA38980.10515672614577129No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC37410.10092132183461529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT3800.0112.300367
CGATCGT1450.0103.11673145
GCTAGCC17000.0100.832141
ACGCGTA1101.2732926E-1190.61773145
TCGTGTT18450.085.314154
CGTCGAC2400.081.61728
CTTTCGT19400.080.423871
TTCGTGT20650.076.2157443
GTTCGCG1600.074.320933
TTACGCG1500.069.958556
TTTCGTG25000.069.6721342
AACGATT5150.067.9200069
CGGCGAT1550.067.695432
CGTGTTC23500.067.576975
CGCGACT2350.065.484273
CGCCGTA1600.061.2129067
GCGGATA2650.061.124218145
TTCGCGC1950.060.9886784
GTTAGCG702.988072E-759.970131
CCGATAC4750.058.911678
Produced by FastQC (version 0.11.5)