FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3706848
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2635727.110407548407703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGT12200.054.172029
CGGTTAG4750.054.0336158
TCGCGAG3350.049.7996378
TGCGAGT12350.048.3185239
CGTGACT7650.046.9703989
ACGTAGT7550.045.0429239
CGAGAGT11400.043.3394629
GTCGGAC6850.043.0888528
TCGGACT8050.043.0423059
GCGTAGT15250.042.9168629
GGGGGAC38950.042.8315248
GGGTAGT30800.042.2905129
CGTTAGT6400.042.1082279
CGGCACT10600.041.767739
GGCGGTA10500.041.5544247
GGCGACT20300.040.458679
CGCGATT3550.039.7641759
GCGGTTA6000.039.5683677
CGCGAAC3750.039.3544858
GGGTACT20800.039.177629
Produced by FastQC (version 0.11.5)