FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7086556
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT26200.065.592029
CGCGATT12150.062.008659
CGCGTTA6000.055.0010387
GGGTATT51200.053.63059
CGGTATT23150.053.2546359
CGGGATT29400.052.9463359
GGGGATT75800.051.4219259
CGTTATT19250.049.811919
GCGTAAT15750.049.811919
GCGGGAT19850.047.0515568
ACGGATT15300.046.8004049
GGCGATT25850.046.0061659
TGCGTTA7600.045.879717
CGGTCGA11100.045.4368637
GCGTATT28250.045.4037789
GCGGTAT16200.045.353448
GTCGCGA8200.044.0413327
CGCGGTA8350.043.995867
GGCGGTA22500.043.72417
TCGCGTA5900.043.2691277
Produced by FastQC (version 0.11.5)