FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091075
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8498037.028349423024835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT44250.075.895979
CGGGATT49550.069.8514569
CGCGATT16800.068.418599
ACGGATT23800.064.4838949
GGGGATT149950.063.2933859
GACGATT22150.057.11149
GGCGATT43450.057.046359
CGCGTTA6650.056.9719057
GCGTAAT24700.054.5949759
GGGTATT95700.054.417589
CGGTATT39250.053.1709029
GCGTATT42050.051.768329
GCGCATT31700.051.654879
CGTTATT31400.051.5348749
TGCGATT33100.050.634079
GGCGGAT41500.047.9671558
CGCTAAT16800.047.3961149
GGCGTAT23350.046.751168
GCGGGAT42300.046.6045538
CGATATT29250.045.4438179
Produced by FastQC (version 0.11.5)