FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772915
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT24950.069.5514149
CGCGATT10650.067.891559
CGGGATT31350.061.569889
GGGTATT48750.061.0033959
CGGTATT20800.060.152999
CGCGATA6150.057.2506459
GGGGATT81400.056.6942639
GGCGATT23750.055.3286559
ACGGATT14950.052.9903569
ACGCGAT4500.051.696358
GCGTATT24050.051.501359
GCGTAAT14150.048.876919
CGCGTTA4300.046.789917
CGTTATT15800.046.558219
TCGCGAG6300.045.9079558
GCGGTAT14150.045.322568
GGCGTAT14100.045.037368
GGCGGAT24350.044.9284028
GGGGTAT40300.044.6202168
CGTGCGA9350.043.7091567
Produced by FastQC (version 0.11.5)