FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5920955
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA125070.21123281632777144No Hit
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC105030.17738692491329522No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG90230.1523909572020054No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG65900.11129961298472965No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC63030.10645242194882412No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA60390.10199368176248595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT8400.0102.07137
ACGCGTA3050.087.47001145
GTTAGCG1300.084.412971
CGTCGAC6500.078.082668
GTTCGCG3450.077.532793
CTAGTCG12800.071.8017043
CGATCGT1900.070.20619145
GTACCCG13100.065.447535
GCTAGCC13050.061.4904861
CGCGCTT4900.060.1873139
TCGTGTT19850.060.1213384
TAGTCGA15650.058.2877354
TAGACTC37800.057.880399
AGTCGAA15800.057.7363245
CGAACGT2500.057.611861
GCTTAGA38850.057.556216
CGCCGTA3600.057.1599277
TACCCGT15350.055.8556676
CGGTATA7900.055.5671966
CGAGTTG9400.055.4549525
Produced by FastQC (version 0.11.5)