FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6613276
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA131680.19911462942118247No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG114580.17325755041827984No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA103590.15663946280179444No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG83730.12660896052123033No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC72330.10937090785262855No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG71710.10843339972503793No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC71530.10816121994606002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT9350.0115.293727
CGATCGT1500.0105.23151145
GTTAGCG2500.080.173841
ACGCGTA2850.078.4621145
GCTAGCC22000.077.911891
CGGCGAT3200.071.260692
TACCCGT15000.070.9466556
CGTCGAC6250.070.761848
TCGTGTT26200.069.103364
GTACCCG15850.066.705965
CTTTCGT28250.064.344581
TTACGCG3150.063.619456
CGCACTA4050.061.43591
CGAACGT2700.061.4358941
CTAGTCG13050.058.2449653
TTCGTGT32650.055.8723453
CGATTAG3850.053.8561441
TTTCGTG39700.053.087882
TAGACTC38800.052.7188269
CGTGTTC34750.051.902495
Produced by FastQC (version 0.11.5)