FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8252718
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG148650.18012247601335707No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA131880.15980189799287944No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC104430.12654012896114952No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG102500.12420150549188765No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA94060.1139745717713849No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC85400.10348105920982638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT4000.098.823044145
ACCCGTT6000.087.888947
GTTAGCG3500.086.162111
CGAACGT4100.080.2396551
CGCCGTA4550.078.268447
TTACGCG5100.075.240126
CGCACTA6900.074.497871
CGTTATA8350.069.715169
GTTCGCG4550.069.273993
GCTAGCC23700.068.827231
CGCGCTT6250.062.4582189
ACGTAAC8050.060.4028177
CGCGATA4250.059.6221357
CGGCGAT4650.058.94282
TCGTGTT26250.058.7333564
CGTCGAC8450.058.353998
CTTTCGT27100.055.8926471
GCTCGAT10950.054.454881
CCGACGT11600.054.3452345
TCGCGAA10400.053.9976969
Produced by FastQC (version 0.11.5)