FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8252718
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT30250.061.4221889
CGCGATA10300.053.4998179
CGCGATT14400.052.9853949
CGGTATT21950.051.8186729
CGCGTTA6200.050.1406677
CGGGATT35750.048.415569
ACGGATT17000.045.712899
GCGTAAA23600.045.501589
GCGGATA21450.045.1219839
GCGTAAT14400.044.64519
GCGCATA15950.043.4070559
CGTTAAT13850.042.8473979
TCGCGTA7450.042.6761637
ACGCGAT7400.042.008738
GCGTTAA11550.041.5954178
CGCGGTA15500.041.4800037
GCGTATA13950.041.020959
GGCGGTA28750.040.794297
CGTGATA14750.040.711949
GCGGTAT19700.040.525738
Produced by FastQC (version 0.11.5)