FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6481781
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC166500.25687384377843064No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG90710.13994610431916785No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA89520.1381101891594301No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG87770.13541031392452166No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA81110.12513536017338445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT2850.089.194954145
ACCCGTT8700.088.347317
GTTCGCG4500.084.666573
CTTTCGT23800.075.069761
CTAGTCG15300.073.8471153
CGCGTTC6050.072.748835
TAGTCGA15550.072.660424
AGTCGAA16000.071.028515
GCTAGCC19400.070.764861
CGATTAG3700.069.236641
CCGCGTT6800.065.690094
TCGTGTT27100.065.205294
TTCGTGT27850.063.448833
GTAATAT42050.062.6399571
CGTCGAC8050.062.017988
GTTAGCG2250.061.3069651
AGCGTGA19700.061.0241176
GCGTGAT20000.060.1101467
CGGAGTG21900.059.389672
TTACGCG4200.057.8720786
Produced by FastQC (version 0.11.5)