FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6481781
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3429815.2914623311093045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAC37350.055.041223
GCGGATT15950.054.5735559
GACGATT11350.049.495919
GGGGATT83100.049.1048369
CGTTATT12300.048.1825689
CGGGATT24100.046.6206329
CGCGAGT10300.045.551249
GCGTAAT11150.043.7397239
CGCTAAT7350.043.6756979
GGGTATT46650.043.6702889
CGCGAAT9600.043.0851259
CGGTATT15850.042.8437239
CCGCAGA48050.042.7969362
CGGTAAT16700.041.772059
GGCGATT20700.041.155949
CGCGATT8900.040.924739
CGGTTAG6250.040.4974448
GGCGAAT20450.040.451569
CGTGATT13900.040.4157269
CGGGTTA9750.039.8896037
Produced by FastQC (version 0.11.5)