FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235817
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT19550.053.6365439
CGCGATT10700.050.6663979
CGCGTTA5150.048.480657
GCGTAAT13100.046.284749
CGGTATT18800.045.5316739
TTCGCTA6050.044.8059657
CGCGATA7050.043.5080459
GCGGATA14700.043.187989
CGGGATT26000.043.0741279
CGATCGA5400.042.2730757
CGTTAGT11050.041.9605649
GCGTATA12050.041.4382329
GGCGGAT24650.040.8036848
GCGTATT17200.039.3990149
CGCTAAT11050.039.3783769
GGCGTAT12400.038.5434238
TCGCGTA5950.038.365487
GGCGGTA18900.038.1212547
CGCGAAA12650.037.781099
CGTTCGA5900.037.481537
Produced by FastQC (version 0.11.5)