FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763388
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3321856.973712827928356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT14850.075.455049
CGCGATT5950.064.224939
GGGGATT53900.058.7609339
CGGGATT18300.056.2748879
GGCGATT16500.055.7407729
GGGTATT35500.052.910069
GCGTAAT9250.052.515579
CGACCGA3050.050.9653637
ACGGATT9450.050.0333569
CGGTATT17450.049.7368139
ACGCGAT2400.048.576918
GCGTATT14850.047.5410359
CGTTATT11650.046.144599
CGCGAAT6750.046.0581059
GCGGGAT14400.045.878198
CGGTAGT15000.045.3384489
GGCGTAT9500.044.9975558
CGTGACT9250.044.813299
CGCTAAT6800.044.7669539
GCGGTAT8000.044.5288358
Produced by FastQC (version 0.11.5)