FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7113932
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT27950.061.5369389
ACGGATT12500.060.6444249
CGCGATT10700.058.3439989
GGGGATT83100.052.7401479
CGGGATT31650.052.1290669
GCGTATT21650.050.902859
CGCGTTA5400.049.5464487
GGCGATT25850.047.5609669
GGGTATT55950.047.4777229
TGCGTTA7100.045.7581867
CGGTATT25200.045.7543079
CGTTATT17850.045.679969
GGCGAAT23750.044.5244339
CGTCGAC10450.044.5003628
GCGCATT21850.044.3145189
GACGATT11850.043.5432249
GCGCTAT8700.043.2005968
GCGGGAT24000.042.999248
GGCGGAT25750.042.7982338
GGCGCTA10750.042.665917
Produced by FastQC (version 0.11.5)