FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225147
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2249276.974162728086503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGATT52850.075.067589
GCGGATT11600.071.531629
CGGGATT14800.065.7015469
GGGTATT28800.064.375349
CGCGATT4250.064.062879
GGCGATT12100.061.0752839
GACGATT5750.057.497429
CGTGCGA3250.055.849687
CGGTATT9800.053.580289
GGCGAAT11400.051.746699
GGGGTAT28250.051.6309478
GGCGGAT11350.051.4034968
ACGGATT6100.051.0102469
ACGGGAT6950.049.4352958
GCGTATT8500.048.8098079
CGTGATT7100.046.564819
GGGGGAT56000.046.1881878
GGGGCGA30900.046.1540957
GGGGGTA43950.045.1344957
CGATATT7350.044.9809769
Produced by FastQC (version 0.11.5)