FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005724964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284465
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG103770.24220060147533007No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA91130.2126986683284844No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG66210.15453504696619064No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC62010.14473218943321978No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA61570.14370522340595618No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG61510.1435651825840566No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC60320.14078770628304818No Hit
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC54640.12753050847655426No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT45100.10526401779452045No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA43790.10220645984971287No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC43660.10190303806893043No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG43140.1006893509458007No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA42900.10012918765820236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)