FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284465
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3009317.023770762510605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT17700.083.857839
CGCGATT6000.074.963819
CGGGATT21250.070.151029
GGGGATT59050.067.6823359
GGCGATT17800.065.337649
CGCGAAT5750.064.813439
ACGGATT9600.063.5853779
GGGTATT34300.061.2573979
CGCGTTA2350.057.419097
CGGTATT12100.056.2892349
GACGATT7100.056.1097349
GCGGGAT15950.054.3848578
GCGTATT13300.054.1092229
GCGCATT11900.053.995549
GGCGGAT17300.053.1122638
GGGCGAT16800.049.7208988
GGCGTAT9000.049.2619368
TGCGATT11750.049.2163629
CGGGAAT19500.049.097189
GCGAATT16050.048.8415769
Produced by FastQC (version 0.11.5)