FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7552083
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4029555.335680235505886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT25750.073.2374349
CGCGATT12700.067.800769
GGCGATT30150.060.1359069
CGGGATT31850.058.449249
GGGGATT101300.056.3287549
GGGTATT48050.056.0324449
GCGTAAT13350.055.869379
CGCGAGT14550.055.4291959
GACGATT13650.054.6414769
CGGTATT18850.054.365749
CGCAGAC40250.051.526963
GGCGAAT31450.051.2874349
CGCGAAT12350.051.064189
CCGCATT11700.050.79149
GCGCATT20300.050.7811859
ACGGATT17750.050.5607269
GGCGGAT26150.047.768938
CCCGATT11550.045.675919
CGGGAAT34200.045.5676849
TGCGATT16450.045.3407979
Produced by FastQC (version 0.11.5)