FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8942341
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT15150.069.830389
GCGGATT34100.068.8010569
CGGGATT39400.059.8619889
GGCGATT36850.056.9115649
CGGTATT32350.055.594489
CGTTATT21650.055.4763079
GGGTATT61950.055.4505549
GCGTAAT16650.054.1953359
ACGGATT17300.053.5979659
GGGGATT100750.053.3674939
CGCGTTA6150.051.6063547
GCGGGAT26900.051.1267478
GCGGTAT18550.049.315288
GACGATT15050.047.1385779
TCGCGAG6950.046.5614668
GGCGGAT29650.046.3848048
ACGCGAT6850.046.332718
CGTGATT26050.045.8671349
GCGAATT30250.045.6704989
GCGTATT32000.044.923149
Produced by FastQC (version 0.11.5)