FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7660059
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA157980.20623862035527404No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG117020.15276644736026185No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA105150.13727048316468582No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG85550.11168321288386944No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC79050.10319763855604767No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG78690.10272766828558369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG2600.0100.809191
ACCCGTT7050.0100.7570957
GTTCGCG3600.082.371393
CGATCGT1601.8189894E-1274.93709145
GCTAGCC23450.071.474861
TCGCGCT4550.065.175535
CGCCGTA4150.064.8102957
ACGCGTA3400.062.69247145
TTACGCG3950.061.108056
CGAACGT2900.059.4609571
CGTCGAC6100.057.6606038
TTCGCGC5150.057.581894
CGCACTA5500.056.4338571
CGTATCG6450.055.767136145
TCGTGTT27600.055.2214554
TAGACTC46150.054.2467549
GCTTAGA47950.052.928156
GCGATTA3850.051.9475146
CGCAATC6250.051.8611952
AGCGTGA17800.051.529886
Produced by FastQC (version 0.11.5)