FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143714
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2910337.02348183296434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT16000.076.97639
CGGGATT17250.066.5131769
ACGGATT8050.065.224789
GGGGATT50950.064.249639
GGCGATT15650.063.7866639
CGCGATT6050.063.2159
GCGCATT9700.058.807739
GACGATT7300.058.6063359
CGGTATT13700.057.7248429
GCGTATT13200.056.473859
CGCGTTA2100.055.561857
GGGTATT31700.053.3709269
CGCTAAT5050.052.627899
CGCGAAT6500.051.857739
GCGGAAT13450.051.5685589
CGTTATT10200.050.840919
GCGGGAT14850.050.635498
GCGCAAT8550.050.0381559
GGCGTAT8850.049.8068548
CGTTAAT6800.049.5698859
Produced by FastQC (version 0.11.5)