FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6669606
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4721107.078529076530157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT29050.078.532119
GGCGATT28150.072.736579
CGGGATT31950.072.195189
CGCGATT11200.071.6591959
GGGGATT89950.068.42969
GGGTATT52700.067.7523049
CGGTATT21750.064.7935649
ACGGATT15450.063.4000249
GCGGGAT24450.059.447728
GCGTATT20750.059.3885549
GCGTAAT16000.059.245799
GACGATT10900.057.397769
CGCGAAT11800.055.697749
GGCGAAT25150.054.5265469
GGCGGAT26350.054.4416738
CGCGTTA3600.054.4183547
CGCAGAC32100.054.347733
CGTGCGA8800.053.859817
TGCGATT17500.053.8064659
GGCGTAT12600.053.6660278
Produced by FastQC (version 0.11.5)