FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048274
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2136147.007703375746406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT3750.073.785649
CGGGATT11550.061.8408789
GGGGATT39550.058.572089
CGATCGA1800.057.1981667
GCGGATT8500.056.7775889
GGGTATT24300.055.8741349
GGCGATT9000.055.0532349
GCGTATT8550.052.682529
CGGTATT9400.051.3414429
GACGATT5150.049.978989
GCGATAT4700.049.2877858
ACGTAGT5650.048.9727639
GCGGGAT9200.048.2609568
GCGCATT8600.047.8868379
CGCGAAT4200.047.4949079
GGCGAAT7900.046.4282579
CGCGACT4300.046.3903739
CGTGATT7600.045.72099
ACGGATT6600.044.8485649
GTACGGA3300.044.8485647
Produced by FastQC (version 0.11.5)